Simple kinetics, assay, and trends for soil microbial catalases
نویسندگان
چکیده
منابع مشابه
Rapid and simple kinetics screening assay for electrophilic dermal sensitizers using nitrobenzenethiol.
The need for alternatives to animal-based skin sensitization testing has spurred research on the use of in vitro, in silico, and in chemico methods. Glutathione and other select peptides have been used to determine the reactivity of electrophilic allergens to nucleophiles, but these methods are inadequate to accurately measure rapid kinetics observed with many chemical sensitizers. A kinetic sp...
متن کاملMicrobial diversity and soil functions
Soil is a complex and dynamic biological system, and still in 2003 it is difficult to determine the composition of microbial communities in soil. We are also limited in the determination of microbially mediated reactions because present assays for determining the overall rate of entire metabolic processes (such as respiration) or specific enzyme activities (such as urease, protease and phosphom...
متن کاملSoil Microbial Biomass Determination
Basic Idea: The quantity of microbial biomass in a given soil sample is inherently difficult to measure. To some degree, any measurement of microbial biomass is relative – different methodologies or variations in methodologies will yield microbial biomass estimates that are not directly comparable. For this reason, if you want to measure microbial biomass – choose a method and stick with it exa...
متن کاملA new assay for tRNA aminoacylation kinetics.
An improved quantitative assay for tRNA aminoacylation is presented based on charging of a nicked tRNA followed by separation of an aminoacylated 3'-fragment on an acidic denaturing polyacrylamide gel. Kinetic parameters of tRNA aminoacylation by Escherichia coli AlaRS obtained by the new method are in excellent agreement with those measured by the conventional method. This assay provides sever...
متن کاملMathematical Models for Microbial Kinetics in Solid-State Fermentation: A Review
Context:In this review, we discuss empirical and stoichiometric models, which have been developed recently in SSF processes and the influence of environmental conditions on the variables of these models. Additionally, new studies on modeling of product formation are also mentioned. <span style="color: windowtext...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2020
ISSN: 0003-2697
DOI: 10.1016/j.ab.2020.113901